Iba strep tag manual

Strep-Tactin®. Like Strep-tag®II, the Twin-Strep-tag® tolerates diverse buffer conditions and additives (high salt, detergents, reducing agents, metal ions and chelating agents) making it a universal tag for varying protein properties. IBA’s 3rd generation Strep-tag® system is based on the novel Strep-Tactin®XT Superflow®. Strep-tag®II is the Twin-Strep-tag®, the sequential arrangement of two Strep-tag®II (28 amino acids, WSHPQFEKGGGSGGGSGGSAWSHPQFEK) leads to a further increase in binding affinity under preservation of its reversibility. The development of the Strep-tag® system and its applications are reviewed by Schmidt et al., Reagents compatible with the Strep-tag®:Strep-Tactin® interaction. For an overview of reagents, which have been successfully tested for the purification of Strep-tag®II and Twin-Strep-tag® fusion proteins and their respective concentrations, see www.doorway.ru

2 THE STREP-TAG®- TECHNOLOGY 1. Strep-tag®II Only 8 aa with a neutral aa composition Rapid one-step purification under physiological conditions emoval necessaryNo tag r 2. Strep-Tactin® Purification of Strep-tag®II and Twin-Strep-tag® proteins Detection of Strep-tag®II and Twin-Strep-tag® proteins High purity due to low tendency of Strep-Tactin® for unspecific. Strep-tag®II is the Twin-Strep-tag®, the sequential arrangement of two Strep-tag®II (28 amino acids, WSHPQFEKGGGSGGGSGGSAWSHPQFEK) leads to a further increase in binding affinity under preservation of its reversibility. The development of the Strep-tag® system and its applications are reviewed by Schmidt et al., Twin-Strep-tag® is a sequential arrangement of two Strep-tag®II sequences with an internal linker region (total size of 28 aa). This tag enables the same mild and rapid purification as Strep-tag®II but, in addition, has an increased affinity for Strep-Tactin® and Strep-Tactin®XT.

The Twin-Strep-tag® and Strep-tag®II expression vectors pASK-IBA/pASG-IBA carry the promoter/operator region from the tet A resistance gene and are the state-of-the-art solution for such an inducible expression system [4, 5]. Browse through our Strep-tag application notes, brochures, flyers, posters, protocols and white papers. Download area To be able to use the full range of Shopware 6, we recommend activating Javascript in your browser. Twin-Strep-tag® is a sequential arrangement of two Strep-tag®II sequences with an internal linker region (total size of 28 aa). This tag enables the same mild and rapid purification as Strep-tag®II but, in addition, has an increased affinity for Strep-Tactin® and Strep-Tactin®XT.